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ATAGO Autorotator solves the effects of optical rotation
A fully-automatic polarimeter can be used to determine the rotation angle or optical rotation of a sample solution. Specific optical rotation is an intrinsic property of a substance at a specific temperature and wavelength and can be calculated from the length of the glare tube, rotation angle, and concentration. Optical measurement of pharmaceutical raw materials and finished products used in the pharmaceutical industry, measurement of components in fine chemical industry or of perfume and perfume secondary optically active substances, measurement of the purity of sugars in the food industry or quality of invert sugar, and specific rotation of optically active compounds Or content measurement.
The object is optically active and its structure cannot have the property of invariant spatial inversion. For molecules or solutions in which molecules can be randomly oriented, the molecules must have chirality. The optical rotation caused by the chirality of the organic molecules is called internal rotation light, and the internal rotation light is divided into left-handed light and right-rotatory light, but if an object has both left and right optical rotations inside or the structure of the object molecule itself has opposite chirality, The object does not show optical rotation. Because the inside of an object has both left-handed and right-handed molecules and does not exhibit optical rotation, it is called racemization. However, the structure of the molecule itself has opposite chirality and does not express optical rotation. It is called mesotropy.
How to use the automatic polarimeter: Connect the polarimeter to 220V AC power. Turn on the power switch, and after about 5 minutes, the sodium lamp will glow normally and you can start working. Check whether the zero position of the polarimeter is accurate. That is, when the polarimeter is not placed in a test tube or placed in a test tube filled with distilled water, observe whether the brightness of the field of view is the same at zero degree. If there is an inconsistency, there is a zero error, which should be subtracted or added to the measurement reading. Or relax the cover plate with four screws on the back, and slightly adjust the dial cover to correct it (correction error of only about 0.5°, which should be sent to the manufacturer for repair). Select the appropriate length of test tube, fill the liquid to be tested, install a rubber band, and screw on the nut until it does not leak water. Nuts should not be screwed too tight, otherwise the guard glass will cause stress and affect the correctness of the reading. Then dry the remaining solution on both ends of the test tube so as not to affect the observation clarity and measurement accuracy. Measure optical readings: Rotate the dial, the analyzer, and obtain a consistent brightness in the field of view. Read from the dial. A positive reading is a right-handed substance, and a negative reading is a left-handed substance.
The special configuration of ATAGO automatic polarimeter: